Subcutaneous (SC) injection is a technique for delivering drugs, vaccines, or biological agents into the subcutaneous tissue.

Intravitreal injection is a technique used to deliver drugs, viral vectors, or cells directly into the vitreous humor of the eye.

The calcium imaging protocol involves loading neurons with a fluorescent calcium indicator, stimulating neuronal activity, and capturing calcium transients using fluorescence microscopy

The optical fiber implantation protocol involves surgically implanting an optical fiber into the target brain region to enable precise light delivery for optogenetic stimulation in vivo.

The optical stimulation protocol involves using light to activate optogenetically modified neurons by delivering specific wavelengths through fiber optics or LEDs

Virus infection of primary neurons involves preparing viral stocks, optimizing multiplicity of infection (MOI), exposing neurons to the virus, incubating under controlled conditions.