Cultivation of mouse cerebral cortex neurons involves isolating cortical tissue, enzymatic dissociation, plating on coated culture dishes, and maintaining in Neurobasal medium for neuronal growth

Recombinant lentivirus (LV) production involves transfecting HEK293T cells with packaging plasmids, followed by viral assembly, harvesting, and purification

Production of recombinant AAV (rAAV) involves transfecting HEK293 cells with helper plasmids, followed by viral assembly, purification, and titer determination for gene therapy applications.

Isolation, dissociation, and culture of primary neurons from brain tissue, followed by incubation in Neurobasal medium for maturation and dendrite formation.

The Tissue Culture Infectious Dose 50 (TCID₅₀) assay is a virological method used to determine the concentration of a virus required to infect 50% of cultured cells

The plaque assay is a virological method used to quantify infectious virus particles in a sample. It involves infecting a monolayer of host cells with a diluted virus solution