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Lentivirus (LVs) are single stranded RNA (sRNA) viruses, which are a subgroup of the retrovirus family. They can integrate into the host genome in both dividing and non-dividing cells, with a wide ran
Lentivirus (LVs) are single stranded RNA (sRNA) viruses, which are a subgroup of the retrovirus family. They can integrate into the host genome in both dividing and non-dividing cells, with a wide ran
In contrast to transient expression, stable expression allows long term, as well as defined and reproducible, expression of the gene of interest
A monoclonal cell line is essentially generated from an original polyclonal population, but it is required to separate from the polyclonal pool in order to create a pure, monoclonal cell line that is
Virus infection of primary neurons involves preparing viral stocks, optimizing multiplicity of infection (MOI), exposing neurons to the virus, incubating under controlled conditions.